Abstract: OBJECTIVE To establish an HPLC method for determination of polysaccharides composition and the monosaccharide content from Ginkgo Folium. METHODS The analysis was performed on Agilent Zorbax Carbohydrate (250?mm×4.6 mm, 5 μm) chromatographic column, and the mobile phase was acetonitrile-water(80∶20), the flow rate was 1.0?mL·min^-1 and the column temperature was maintained at 30 ℃. RESULTS The calibration curves were linear within the range of 4.160-20.80 μg for rhamnose, 8.040-40.20 μg for arabian sugar, 4.096-20.48 μg for fructose, 4.000-20.00 μg for mannose, 8.152-40.76 μg for glucose and 3.96-19.8 μg for galactose respectively. The recoveries of rhamnose, arabian sugar, fructose, mannose, glucose, galactose were 99.6%, 100.9%, 99.7%, 99.1%, 98.8%, 100.1%, respectively. CONCLUSION This method is simple and rapid, stability and feasible, it can be used for the quality control of polysaccharide compositions in ginkgo biloba leaves.