Abstract: OBJECTIVE To investigate the intestinal absorption kinetics of nobiletin self-assemble proliposomes(NSAP) in rats. METHODS Compared with nobiletin and nobiletin liposome, the in situ intestinal absorption of NSAP in different intestinal segments was investigated by means of single-pass rat intestinal perfusion technique. RESULTS The results showed that three preparations could be absorbed at each intestinal segment. The cumulative absorptions, K_a and P_eff of three preparations at each intestinal segment appeared NSAP>nobiletin liposome>nobiletin(P<0.05). CONCLUSION NSAP can effectively improve the oral absorption of nobiletin in the intestinal tract.