UPLC测定钩吻素子的含量及其水溶液稳定性

Content Determination and Stability of Koumine by UPLC

摘要: 目的建立超高效液相色谱法测定钩吻素子的含量，并考察其水溶液在室温条件下的稳定性。方法采用Agilent Extend-C₁₈(2.1 mm×100 mm，1.8 μm)色谱柱，以乙腈-0.2%正丁胺水溶液(30∶70)为流动相，流速0.5 mL?min^-1，检测波长256 nm，测定3个批次钩吻素子的含量，并考察钩吻素子水溶液在室温条件下稳定性。结果钩吻素子分离度良好；在10.19~112.2 μg?mL^-1内线性关系良好(r=0.999 7)，定量限为0.1 μg?mL^-1；平均回收率为100.4%(RSD=0.45%，n

Abstract: OBJECTIVE To establish a ultra high performance liquid chromatography(UPLC) method for determination of koumine and to study the stability of koumine solution at room temperature. METHODS The analysis was achieved by Agilent Extend-C₁₈ chromatogram column(2.1 mm×100 mm, 1.8 μm). The mobile phase composed of acetonitrile-0.2% butylamine (30∶70) at flow rate of 0.5 mL?min^-1. The detection wavelength was set at 256 nm. Three batches of koumine were determined and stability of its solution was examined under room temperature. RESULTS Under the estabilished method, the separation of koumine and its main related substances was good. The linear range of koumine was 10.19-112.2 μg?mL^-1(r=0.999 7) with detection limit of 0.1 μg?mL^-1. The average recovery was 100.4% (RSD=0.45%, n=9). The average concentration of koumine were 99.97%(n

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