HPLC-ELSD测定威灵仙中齐墩果酸的含量

Determination of Oleanolic Acid in Weilingxian by HPLC-ELSD

摘要: 目的建立威灵仙中齐墩果酸的高效液相色谱-蒸发光散射法测定方法。方法采用 SumFire TM C18(4.6 mm×250 mm，5 μm)色谱柱，柱温40 ℃；乙腈-水(85∶15)为流动相，流速1.0 mL·min-1；蒸发光散射检测器漂移管温度85 ℃，空气流速2.3 L·min-1。结果齐墩果酸在0.214～1.92 μg内线性关系良好，r =0.999 9，平均回收率为98.8%，RSD为0.9%(n=6)。结论该方法简便，快速，准确，可作为威灵仙的质量控制。

Abstract: OBJECTIVE To establish an HPLC-ELSD method for the determination of oleanolic acid in Weilingxian. METHODS Using SumFireTM C18 column(4.6 mm×250 mm，5 μm) at 40 ℃，acetonitrile -water(85∶15) was used as the moble phase，the flow rate was 1.0 mL·min-1. An evaporative light-scattering detector(ELSD) was used as detector with gas flow rate of 2.3 L·min-1 and drife tube temperature of 85 ℃. RESULTS The oleanolic acid had a good linearity relationship between 0.214-1.92 μg(r=0.999 9). The average recovery was 98.8% with RSD = 0.9%(n=6). CONCLUSION The method is simple，rapid and accurate，suitable for the quality control of Weilingxian.