广西不同产地壮药玉叶金花中Mussaendoside G的含量测定

Determination of Mussaendoside G in Mussaenda Pubescens in Different Areas of Guangxi Province by HPLC

摘要: 目的建立高效液相色谱法测定玉叶金花中mussaendoside G的含量。方法色谱柱为Techmate C₁₈(4.6 mm× 250 mm，5 μm)，流动相：乙腈-水(38∶62)，检测波长：265 nm，流速：1.0 mL·min^-1。结果 mussaendoside G在0.06~ 0.60 mg·mL^-1(r=0.999 9)内线性关系良好，平均回收率为99.34%，RSD为2.32%。不同产地玉叶金花中mussaendoside G的含量相差较大。结论此方法简便、快捷、准确，可用于玉叶金花药材的质量控制。

Abstract: OBJECTIVE To establish an HPLC method for the simultaneous determination of mussaendoside G in Mussaenda pubescens in different areas of Guangxi province. METHODS Mussaendoside G was separated on Techmate C₁₈ (4.6 mm×250 mm, 5 μm) column and detected at 265 nm. The mobile phase was acetonitrile-water(38∶62). The flow rate was 1.0 mL·min^-1. RESULTS Mussaendoside G was 1inear within the range of 0.06-0.60 mg·mL^-1(r=0.999 9). The average recovery was 99.34%(RSD=2.32%). The contents of mussaendoside G in different areas were different. CONCLUSION The method is simple, rapid, and it can be used for the quality control of Mussaenda pubescens．