HPLC测定注射用亚锡依替菲宁的含量

Determination of Etifenin and Stannous Chloride for Injection by HPLC

摘要: 目的建立用HPLC测定注射用亚锡依替菲宁(EHIDA药盒)含量的方法。方法采用Waters C₁₈(4.6 mm×150 mm，5 μm)的色谱柱，以甲醇-水-磷酸(55∶45∶0.075)为流动相，检测波长为220 nm，流速1.0 mL·min^-1。结果依替菲宁在32~384 μg·mL^-1内成线性关系，Y=1 789 041X+91 432(r=0.999 92)平均回收率为98.77%，RSD为0.43%。结论本方法操作简便，结果准确，可用于EHIDA药盒含量的快速定量分析。

Abstract: OBJECTIVE To establish a method for determination of etifenin and stannous chloride for injection(EHIDA kit). METHODS HPLC was adopted with Waters C₁₈(4.6 mm×150 mm, 5 μm) column, methanol-water-phosphoric acid (55∶45∶0.075) was used as the mobile phase. The flow rate and detection wavelength was 1.0 mL·min^-1, 220 nm, respectively. RESULTS The linear rage of etifenin were 32-384 μg·mL^-1, r=0.999 92, the average recovery was 98.77%, RSD was 0.43%(n=7). CONCLUSION The method is simple, accurate and suitable for the determination of EHIDA kit.