Abstract: OBJECTIVE To study the effect of the processing methods on the content of diacrylate alkaloids of Radix Aconiti (RA). METHODS The RA was processed,using Ch.P,Black Bean and Vinegar law metheds.RP-HPLC with gradient elution was established for determining the content of diacrylate alkaloids(mainly aconitine、hypaconitine).RESULTS Three processing methods can significantly reduce the contents of aconitine and hypaconitine. Chromatographic methods established can be applied to the quantitative analysis of the diacrylate alkaloids of processed products.CONCLUSION The described processing methods can reduce the content of diacrylate alkaloids of RA.