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    姜黄醋制工艺优化及其对寒凝血瘀型痛经大鼠的药效作用研究

    Optimization of Vinegar Processing Technology of Curcumae Longae Rhizoma and Its Pharmacodynamic Effect on Dysmenorrhea Rats with Cold Coagulation and Blood Stasis Type

      摘要
    • 摘要:
      目的  优化姜黄醋制工艺，探讨醋制前后姜黄对寒凝血瘀型痛经大鼠的药效作用。
      方法 以炒制温度、炒制时间、加醋量、闷润时间为考察因素，姜黄素、去甲氧基姜黄素、双去甲氧基姜黄素、多糖、炮制饮片性状作为综合评价指标，运用多指标综合评价并结合熵权法-层次分析法等数学方法进行评价，Box-Behnken响应面法优化炮制工艺。将56只SD大鼠随机分为空白组，模型组，阳性药组(0.018 g·kg−1)，生品高、低剂量组(0.54、1.08 g·kg−1)，醋制品高、低剂量组(0.54、1.08 g·kg−1)。采用冰水浴联合苯甲酸雌二醇以及缩宫素建立寒凝血瘀型痛经大鼠模型。检测扭体反应、血液流变学指标、观察HE染色子宫组织病理形态变化、ELISA法测定大鼠血清中PGE2、IL-6、AVP、OT、β-EP含量。
      结果 最佳醋制条件为炒制温度100 ℃、炒制时间15 min、加醋量40%、闷润时间2 h。综合评分平均值为90.49，与预测值90.1421相差<1%，符合预测值。姜黄醋制后能显著降低扭体次数，延长扭体潜伏期(P<0.01)，提高扭体抑制率；显著降低全血黏度低切、中切、高切值(P<0.01)和血浆黏度(P<0.01)。子宫HE染色组织病理形态观察发现，醋制姜黄给药后可改善大鼠子宫病变程度，且高剂量组效果优于低剂量组。ELISA结果显示，醋制姜黄可显著降低PGE2、IL-6、AVP、OT含量(P<0.05或P<0.01)，显著升高β-EP含量(P<0.05或P<0.01)。
      结论 该醋制姜黄工艺稳定，操作简便可行，且相较生品对寒凝血瘀型痛经大鼠的药效作用更强。

       

      Abstract:
      OBJECTIVE  To explore the pharmacodynamic effect of vinegar-processed Curcumae Longae Rhizoma on dysmenorrhea rats with cold coagulation and blood stasis through optimization of its vinegar processing technology.
      METHODS Taking frying temperature, frying time, adding vinegar amount and moistening time as factors, the properties of curcumin, demethoxy curcumin, double demethoxy curcumin, polysaccharide, and the characteristics of processed herbal slices were used as comprehensive evaluation indexes, and the multi-index comprehensive evaluation combined with entropy weight method-hierarchy method and other mathematics were used to evaluate the optimization of processing technology by Box-Behnken response surface methodology. 56 SD rats were randomly divided into blank group, model group, positive drug group (0.018 g·kg−1), raw product high-low dose group (0.54, 1.08 g·kg−1) and vinegar product high-low dose group (0.54, 1.08 g·kg−1). The rat model of dysmenorrhea of cold coagulation and blood stasis type was established by ice water bath combined with benzoic acid and oxytocin. Detection of hemorheological indexes of writhing reaction and observation of pathological changes of uterine tissue stained by HE. Determination of PGE2, IL-6, AVP, OT, β-EP content in serum of rats by ELISA method.
      RESULTS  The conditions of vinegar preparation are as follows: stir-frying temperature 100 ℃, stir-frying time 15 min, adding vinegar amount 40% and stuffing time 2 h. The difference between the average score of 90.49 and the predicted value of 90.1421 was<1%, which accorded with the predicted value. Curcumae Longae Rhizoma vinegar could significantly prolong the latency of writhing (P<0.01) and increased the inhibition rate of writhing. The whole blood viscosity was significantly low shear, middle shear and high shear (P<0.01) and plasma viscosity (P<0.01). Uterine histopathologic observation of HE staining showed that the high-dose group was better than the low-dose group in improving the degree of uterine lesions. The results of ELISA showed that the content of PGE2, IL-6, AVP, OT was significantly increased (P<0.05 or P<0.01) and the content of β-EP was significantly increased (P<0.05 or P<0.01).
      CONCLUSION  The process of making Curcumae Longae Rhizoma with vinegar is stable, simple and feasible. Compared with raw products, the drug effect on dysmenorrhea rats with cold coagulation and blood stasis type is stronger.

       

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