Abstract: OBJECTIVE To study the spectrum-activity relationships between the HPLC fingerprints and the regulation of gastrointestinal motility activity of flavonoids in Qu Aurantii Fructus methanol extract. METHODS The models of intestinal carbon propulsion and contraction models of isolated ileum were established to obtaine the efficacy results of regulating gastrointestinal motility. The LC-MS was used to identify the components of Qu Aurantii Fructus methanol extract. Multivariate regression analysis(MRA) and bivariate correlation analysis(BCA) were used to associate efficacy data of Qu Aurantii Fructus methanol extract with the relative peak area of the fingerprint common peak to analyze the spectral effect correlation. RESULTS The 12 components were identified by LC-MS, studied by MRA and BCA, to obtain the spectrum efficiency equation (Y₁=52.379-0.255X₂+0.335X₃-4.499X₄+4.308X₅+0.750X₆, R²=0.959; Y₂=0.250+0.013 5X₂+0.023 0X₃+0.076 9X₆, R²=0.921). It was showed five peaks were the correlated peaks, and 2 of them were significantly correlated. After fitting verification for different samples substituted into the spectrum-activity equation, the fitting error between the predicted value and the measured value of the fitting equation was <3%. CONCLUSION Narirutin(peak 2), naringin(peak 3), naringenin(peak 4), hesperidin(peak 5) and neohesperidin(peak 6) in Qu Aurantii Fructus methanol extract may be the material foundation in Qu Aurantii Fructus methanol extract for regulation of gastrointestinal motility, in which naringin(peak 3) and neohesperidin(peak 6) are significant correlation.