| 引用本文: | 孙荣飞,张金玺,赵晓星,者从章.百贝益肺胶囊含量测定的方法改进[J].中国现代应用药学,2017,34(12):1740-1742. |
| SUN Rongfei,ZHANG Jinxi,ZHAO Xiaoxing,ZHE Congzhang.Improvement of Content Determination of Baibei Yifei Capsule[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(12):1740-1742. |
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| 摘要: |
| 目的 增加评价指标，建立科学、合理的百贝益肺胶囊含量测定方法。方法 C18小柱纯化样品，优化色谱条件为：Vp-ODS色谱柱（150 mm×4.6 mm，5 μm）；柱温：20℃；变动流速；流动相：乙腈（A）-水（B），梯度洗脱；进样量：10 μL；检测波长：203 nm。结果 所测成分三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1分别在40.24~241.44（r=0.999 6），91.50~549.00（r=0.999 9），35.72~241.32（r=0.999 8）μg·mL-1内线性关系良好；加样回收率为94.15%~99.81%，RSD为1.12%~1.94%。结论 该方法准确，重复性良好，可为修订标准提供依据。 |
| 关键词: 百贝益肺胶囊 含量测定 改进 |
| DOI：10.13748/j.cnki.issn1007-7693.2017.12.021 |
| 分类号:R917.101 |
| 基金项目: |
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| Improvement of Content Determination of Baibei Yifei Capsule |
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SUN Rongfei1, ZHANG Jinxi1, ZHAO Xiaoxing1, ZHE Congzhang2
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1.Zhaotong Institute of food and drug control, Zhaotong 657000, China;2.YongZitang Pharmaceutical Company Limited, Zhaotong 657000, China
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| Abstract: |
| OBJECTIVE To augment evaluation index, and establish a scientific and reasonable method for the content determination of Baibei Yifei capsule. METHODS Samples were purified by C18 column, optimized chromatographic conditions:Vp-ODS column (150 mm×4.6 mm, 5 μm); column temperature:20℃; fluctuan flow rate; the mobile phase acetonitrile (A)-water (B), gradient elution. The injection volume was 10 μL. The detection wavelength was 203 nm. RESULTS The contents of notoginsenoside R1, ginsenoside Rg1, ginsenoside Rb1 were linear in 40.24-241.44(r=0.999 6), 91.50-549.00 (r=0.999 9), 35.72-241.32(r=0.999 8)mg·mL-1, respectively. The recovery rate was 94.15%-99.81%, RSD was 1.12%-1.94%. CONCLUSION The method is accurate and reproducible, which can provide the basis for the revision of the standard. |
| Key words: Baibei Yifei capsule content determination improvement |
