Abstract: OBJECTIVE To investigate and analyze the use of adrenaline in the treatment of anaphylactic shock. METHODS Investigated the injection method and the dose of the adrenaline for treatment of the anaphylactic shock by questionnaire survey on clinical staff. Combined with the relevant clinical guidelines, consensus and literatures such as the adrenaline drug instruction, "First Aid Guide on Severe Allergic Response" and "Experts Consensus on Diagnosis and Treatment of Perioperative Allergic Reaction" etc, the application of adrenaline in the first aid for anaphylactic shock was discussed. RESULTS The results of the study showed that 34% of clinicians and 42% of nurses were advised to use subcutaneous injection when using adrenaline to treat anaphylactic shock in clinic; 20% of clinicians and 30% of nurses thought that intramuscular injection should be preferred. The number of clinicians and nurses who received intravenous injections after diluting adrenaline injections was 20% and 16%, respectively, while the proportion of clinicians and nurses who chose direct intravenous injection without diluting was 26% and 12%, respectively; 70% of clinicians and 76% of nurses believed that a single dose of adrenaline was preferably 0.5 to 1.0 mg. According to the relevant guidelines and literatures, the intramuscular injection of adrenaline was convenient and timely when the anaphylactic shock reaction occurs. It also had a faster absorption rate and a lower risk. It was the first recommended method of administration with a dose of 0.3-0.5 mg. However, adrenaline was directly injected intravenously without dilution, which might cause a sharp rise in blood pressure of the patient, and it might induce arrhythmia and cerebrovascular accident. CONCLUSION It is necessary to strengthen training of rational drug use. In the treatment of anaphylactic shock, a safe and effective administration of adrenaline injection should be selected to promote its rational use.