Abstract: OBJECTIVE To research the therapeutic effect of puerarin solid lipid nanoparticles(Pue-SLN) on liver injury in rats, prepare Pue-SLN and investigate drug release of Pue-SLN. METHODS Pue-SLN was prepared by emulsification ultrasonic dispersion method. Acute liver injury was induced by intraperitoneal injection of carbon tetrachloride in rats. Rats were randomly divided into 6 groups: normal control group, model group, glycyrrhizinate acid diamine positive control group(13.5 mg·kg^-1), Pue-SLN groups with high concentration(27 mg·kg^-1), middle concentration(13.5 mg·kg^-1) and low concentration (6.75 mg·kg^-1). ALT, ALP, AST in serum and MDA, SOD, GSH-PX in liver tissue homogenate were detected. And then, liver index and obtained histomorphology examination of liver tissue were calculated. RESULTS Pue-SLN groups could significantly inhibit the activities of ALT, AST, ALP in serum, decrease the content of MDA in liver homogenate and increase the activities of SOD, GSH-PX in liver homogenate. Pue-SLN groups could improve the pathological of liver tissue via observing confirm the pathological section histomorphology of liver tissue. CONCLUSION Pue-SLN have therapeutic effects on acute liver injury induced by carbon tetrachloride in rats.