Abstract: OBJECTIVE To prepare desvenlafaxine succinate sustained-release tablets, determine the best prescription and preparation method, and study its in vitro release property. METHODS Establishing UV spectrophotometry method determine the in vitro release degree of desvenlafaxine succinate sustained-release tablets. With vitro release as index, we determine the best prescription and preparation process through single factor investigation. RESULTS The best prescription and preparation process were as follows: HPMC K15M was matrix materials, amount of 50%; MCC was filler; magnesium stearate was a lubricant, amount of 8%; preparing wet granules; compressing tablets with pressure of 70 kN. The desvenlafaxine succinate sustained-release tablets had good release property in vitro, and could release drug for 24 h, which met the testing requirements. CONCLUSION The preparation progress of desvenlafaxine succinate sustained-release tablets is stable and reliable, and the in vitro release determination method is simple.