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    HPLC同时测定猫豆中左旋多巴及其衍生物含量

    Simultaneous Determination of L-dopa and Its Derivates in Seeds of Mucuna Pruriens Var. Utilis by HPLC

      摘要
    • 摘要: 目的 建立同时测定猫豆中左旋多巴(2)及其3种衍生物含量的高效液相色谱法。方法 从猫豆中分离获得3-羧基-6,7-二羟基-1,2,3,4-四氢异喹啉( 1 )、1-甲基-3-羧基-6,7-二羟基-1,2,3,4-四氢异喹啉( 3 )、1,1二甲基-3-羧基-6,7-二羟基-1,2,3,4-四氢异喹啉( 4 )等3种成分；含量测定采用Cosmosil C18-PAQ色谱柱(4.6 mm×250 mm，5 mm)，流动相1%乙酸等度洗脱，流速0.5 mL·min-1，柱温30 ℃，检测波长280 nm。结果 左旋多巴( 2 )和3个化合物色谱分离良好，分别在0.298 4~5.968 mg ( 1 )，6.554~131.1 mg ( 2 )，0.262 6~5.252 mg ( 3 )，0.256 8~5.136 mg ( 4 )线性关系良好(r>0.999)，平均加样回收率依次为97.7%，99.1%，98.7%，97.9%。结论 本方法简便、准确，可用于同时测定猫豆中的左旋多巴及其四氢异喹啉类衍生物含量，为其质量控制方法提供参考。

       

      Abstract: OBJECTIVE To establish a method for simultaneous determination of both L-dopa( 2 ) and its derivates in seeds of Mucuna pruriens var. utilis Burck by HPLC. METHODS Three ingredients, 3-carboxy-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline( 1 ), 1-methyl-3-carboxy-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline( 3 ) and 1,1-dimethyl-3-carboxy- 6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline( 4 ) were isolated from the herb. Chromatographic separation was performed on a Cosmosil C18-PAQ analytical column (4.6 mm×250 mm, 5 mm) with 1% acetic acid as the mobile phase at 30 ℃. The detection wavelength was 280 nm and the flow rate was 0.5 mL·min-1. RESULTS The 4 components were separated with satisfaction. Their linearity ranges were between 0.298 4-5.968 mg ( 1 ), 6.554-131.1 mg ( 2 , L-dopa), 0.262 6-5.252 mg ( 3 ) and 0.256 8-5.136 mg ( 4 ), respectively with their correlation coefficients all >0.999. Their average recovery rates were 97.7%, 99.1%, 98.7% and 97.9%. CONCLUSION The method is accurate and selective for simultaneous determination of 4 ingredients in seeds of Mucuna pruriens, which could be applied for the quality control of this herb.

       

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