正交试验优化桂枝总有机酸乙醇回流提取工艺

Optimization of the Extracting Process of Total Organic Acid from Cassia Twig by Orthogonal Experiment with Ethanol Reflux

摘要: 目的考察得到较优的桂枝总有机酸乙醇回流提取工艺。方法用正交设计试验，以总有机酸提取率为指标，考察乙醇浓度、料液比、提取次数3个因素对提取结果的影响。结果较优的提取工艺为乙醇浓度90%，料液比1∶8，提取次数3次。结论该工艺所得桂枝总有机酸提取率较高，工艺稳定、重复性好，适用于桂枝总有机酸的提取。

Abstract: OBJECTIVE To study the optimum extraction technology of total organic acids in Cassia Twig. METHODS Using total organic acid extraction rate as index to investigate the effection of the extraction results by ethanol concentration, dosage of ethanol, extraction times with orthogonal design test. RESULTS Optimum extraction process were as follows: ethanol concentration of 90%, 8 times solvent dosage, extraction for 3 times. CONCLUSION The process proceeds higher extraction yield, stability, repeatability is good, suitable for the extraction of total organic acids.