Abstract: OBJECTIVE To provide information for the rational application of antibiotics, the distribution and drug resistance variance of gram-positive bacteria in hospital were investigated. METHODS The identification of strains and the drug susceptibility testing were performed using a VITEK-2 Compact system. A principal component analysis(PCA) model was established with the use of SIMCA-P+ software. The principal component scores plot in combination with principal component loading plot was applied to monitor the drug resistance variance of gram-positive bacteria. The bacterial strains, which showed significant upward trend in drug resistances, were screened. RESULTS A total of 5?436 strains of gram-positive bacteria were isolated in 2011-2014, including staphylococcus aureus(59.1%), enterococcus faecalis(12.3%), staphylococcus haemolyticus (12.2%), staphylococcus epidermidis(10.3%), staphylococcus hominis(3.7%) and staphylococcus capitis(2.3%). Among these, the drug resistance rates of staphylococcus hominis, staphylococcus aureus and staphylococcus haemolyticus to clindamycin showed significant upward trend. CONCLUSION This study demonstrates that the management of clindamycin should be strengthened by hospital. The monitoring of drug resistance rates of staphylococcus to clindamycin should also be further strengthened.