Abstract: OBJECTIVE To develop an HPLC method for simultaneous determination of chlorogenic acid, caffeic acid and vanillic acid in each fraction of Sargentodoxae Caulis. METHODS The analysis was carried on a column of Kromasil C₁₈(4.6 mm×250 mm, 4.5 μm). Isocratic elution, mobile phase consisted of solvent A (1%, solution of HAc in water) and solvent B (methanol) at the ratio of 68∶32, the flow rate was 1.0 mL.min^-1, the injection volume was 20 μL, the detection wavelength was 327 nm, the column temperature was at 30 ℃. RESULTS Ethyl acetate fraction had the highest concentration of chlorogenic acid (4.52%) and caffeic acid (0.11%) and dichloromethane fraction possessed the highest concentration of vanillic acid of 5.8%. CONCLUSION This method is simple, accurate, rapid and suitable for the usual quality control of Sargentodoxae Caulis.