不同炮制方法对玳瑁浸出物及氨基酸含量的影响

Study on the Effect of Different Processing Methods on Extracts and Amino Acids in Eretmochelydis Carapax

摘要: 目的测定玳瑁药材浸出物、氨基酸的含量及炮制对结果的影响。方法采用药典规定方法测定浸出物的量。全自动氨基酸分析仪测定经厌氧管充氮密封酸水解后玳瑁中氨基酸的含量。结果炮制后浸出物的量明显提高。玳瑁中氨基酸含量较高，17种单体氨基酸总量达75%，其中酪氨酸、甘氨酸、脯氨酸、亮氨酸等含量较高。结论炮制能明显提高玳瑁浸出物的量，其中砂烫醋淬后显著高于生品。全自动氨基酸分析仪能够很好地测定酸水解后的玳瑁氨基酸的含量。

Abstract: OBJECTIVE To determine extracts and amino acids in Eretmochelydis Carapax. METHODS The extract was determined in methods of Chinese Pharmacopoeia. Eretmochelydis Carapax was hydrolyzed in the anaerobic nitrogen-sealed tube by hydrochloric acid. Total amino acids content was determined by Amino Acid Autoanalyzer. RESULTS The extract was increased significantly with processing. The content of amino acids in Eretmochelydis Carapax was high. The total content of 17 kinds of amino acids was 75%, and the content of tyrosine, glycine, proline, leucine was high. CONCLUSION The extract was increased with processing. There was a significant difference between the extract of vinegar-processed and the extract of crude. The conten of amino acid in Eretmochelydis Carapax can be accurately determined by Amino Acid Autoanalyzer.