Abstract: OBJECTIVE To investigate the clinical application and enhance rational use of structured triglyceride(STG). METHODS This is a retrospective study using formulated STG usage evaluation standard to analysis the application rationality of STG in indications, contraindications, usage and dosage, etc. RESULTS Totally of 368 hospitalized patients administrated with STG were randomly collected from April 1 to May 31st in 2015. The rates of rational use in indications, no contraindications, and dosage were 60.60%, 86.96% and 84.24%, respectively. In addition, other irrational phenomena were also existed, such as irrational use in combination medication (rate 93.48%), inappropriate monitoring and dripping speed in STG transfusion. CONCLUSION The irrational phenomena of STG usage were clearly evident in our hospital. Thus, establishing standardized usage guideline for STG and strengthening the drug intervention is urgently needed to improve the current irrational usage situation of STG.