Abstract: OBJECTIVE To prepare neurospoagioma blue nanoscale coloring agent through inspect the preparation quality and screen the prescription, to make it much easier to through hemato encephalic barrier and absorbed by tumor cells. METHODS Phospholipid, NH₂-PEG-NH₂, genipin and fetal bovine serum were used as raw materials, prepared into blue nano coloring agent lipoprotein lyophilized powder by means of ultrasonic homogenization, and the preparation was screened through morphology and particle size. RESULTS The optimum process prescription was 60 mg soya bean lecithin, 900 μL fetal bovine serum, 10 mg·mL^-1 NH₂-PEG-NH₂ and 1.5 mg·mL^-1 genipin; the average particle size was 71.57 nm, the average potential was 49.6 mV, and it was in good shape under the electric mirror. CONCLUSION The particle size and morphology of the prepared blue nanoscale coloring agent lyophilized powder meet the quality requirement and the nano protein can effectively naked eye tracking glioma. This process method is simple and practica.