Abstract: OBJECTIVE to develop a determinative method analyzing content of α-pinene in standards myrtle submicroemulsion with utilizing the static headspace gas chromatography method. METHODS Static headspace condition: headspace heating oven temperature 90 ℃, loop temperature 100 ℃, the transmission line temperature 110 ℃, GC cycle time of 35 min, headspace vial equilibration time 30 min; GC method: column temperature maintained at 50 ℃ for 1 min, then heated from 0.5 ℃·min^-1 to 62 ℃; afterwards from 15 ℃·min^-1 to 180 ℃; FID detector temperature 250 ℃, inlet temperature 230 ℃. a diluted emulsion samples from direct headspace assay α-pinene free concentration was measured after the total concentration of α-pinene while content and entrapment rate of another equivalent sample, which was completely broken, were calculated according to different formulas and standard curve. RESULTS At the concentration degree of 0.001-0.10 μL·mL^-1, the α-pinene has shown good linear relationship, correlation coefficient r=0.999 3. The samples with high, medium, and low concentration of adding rate were recovered at the ratio of 88.44%, 87.39%, 105.62%, respectively. CONCLUSION This analytical method is featured with simple procedure, high sensitivity, and effective selectivity. It thus can be applied to determine content and entrapment efficiency of α-pinene in the emulsion.