Abstract: OBJECTIVE To establish an HPLC method for the assay of propacetamol hydrochloride, and to investigate the compatible stability of propacetamol hydrochloride with 5 kinds of infusion solution. METHODS The variance of propacetamol hydrochloride content in fructose injection, glucose saline injection, 0.9% sodium chloride injection, 5% glucose injection and 10% glucose injection was determined by HPLC. Meanwhile, the changes of color and pH value were observed. RESULTS The content of propacetamol hydrochloride was <90% within 6 h; only the pH value of propacetamol hydrochloride with 0.9% sodium chloride injection was 4.52, and the pH value of the other 4 kinds of infusion solution was near 4 at 0 h; the pH value decreased to <4.0 within 0.5 h in all infusion solution. CONCLUSION Propacetamol hydrochloride is unstable in 5 kinds of infusion solution. The 0.9% sodium chloride injection should be used as preferred infusion solution, and it should be used immediately after configured.