Abstract: OBJECTIVE To establish and improve the quality standard of Xiaobai granules. Methods TLC was used to identify Astragali Radix, Rehmanniae Radix, Polygoni Multiflori Radix, and Psoralen in Xiaobai granules. Astragloside Ⅳ in preparation was determination by HPLC-ELSD on Lichrospher-C₁₈(4.6 mm×250 mm, 5 μm) chromatographic column, the mobile phase was acetonitrile-water(32∶68) at a flow rate of 1.0 mL·min^-1, and column temperature was 25 ℃. RESULTS TLC spots were clear and well-separated without negative interference. The calibration curves of astragaloside Ⅳ demonstrated linear relationship in the range of 0.311 5-2.803 9 μg(r=0.999 9), average recovery rate was 99.4%(RSD=2.67%). CONCLUSION the method established is simple, accurate and reproducible, which can be used for the quality control of Xiaobai granules.