Abstract: OBJECTIVE To improve gynecological perioperative antibiotic prophylaxis by means of antimicrobial stewardship intervention based on the information technology. METHODS Constructing medication rules in perioperative antibiotic prophylaxis and establishing the EMRs embedded antimicrobial stewardship platform, antibiotics preoperative prophylaxis were deliveried directly to preoperation room. Gynecological perioperative antibiotic prophylaxis was compared before and after process intervention based on the above information technology(IT). RESULTS The 586 cases and 532 cases of gynecological surgeries were operated before (Jan-Jun, 2013) and after IT process intervention(Jan-Jun, 2014), respectively. Rate of gynecological perioperative antibiotic prophylaxis was significantly decreased during IT process intervention in 2014 (67.1%) compared to 72.9% in the same period of 2013 before intervention(P<0.05), among which the rate of perioperative antibiotic prophylaxis in type Ⅰ incision gynecological surgeries was significantly decreased from 59.6% to 35.2% (P<0.01). Antibiotics were applied 90.9% in 0.5-2 h before incision. Appropriate antibiotic selection before and after operation were 84.9% and 80.6% respectively in type I incision gynecological surgeries, 99.3% and 94.2% respectively in other type incision gynecological surgeries during IT process intervention in 2014, they were significantly improved with comparison to that of before intervention in 2013 (P<0.01). The duration of perioperative antibiotic prophylaxis was (1.39±0.81)d in 2014, of which 69.2% was not exceed 24 h and 90.2% was not exceed 48 h, was significantly improved with comparison to that of before intervention in 2013. CONCLUSION Antimicrobial stewardship based on the information technology can significantly improve the rational use of gynecological perioperative antibiotic prophylaxis.