Abstract: OBJECTIVE To optimize the chromatographic conditions in the determination and inspection method of Dendrobii Officinalis Caulis in Chinese Pharmacopoeia, and to compare the quality of its produced from different areas. METHODS Based on the contend of Dendrobii Officinalis Caulis from ChP. 2010 to optimize the chromatographic conditions. RESULTS The analyses were performed on Diamonsil C₁₈ columns(250 mm×4.6 mm, 5 μm) with the gradient elution (0~60 min, 14%A; 60~61 min, 14%→25%A; 61~69 min, 25%; 69~70 min, 25%→14%A; 70~80 min, 14%A) of acetamide(A)- 0.02 mol·L^-1 acetonitrile(B) aqueous at a flow rate of 1.0 mL·min^-1. The detection wavelength was set at 250 nm, and the column temperature was controlled at 30 ℃. The injection volume was 10 μL. PMP (1-phenyl-3-methyl-5-pyrazolone) and mannose, PMP and impurity peak, internal standard and impurity peak were all separated successfully using the optimized method. Dendrobii Officinalis Caulis produced from different areas in Zhejiang Province had difference in the content of mannose and inspection item. The content of mannose was the highest(40.8%) in Dendrobii Officinalis Caulis produced from Fuyang. The ratio of inspection item was the the highest(3.9) from Xiaoshan. CONCLUSION The optimized method which is reproducible and accurate reliable can be used for the quality control of Dendrobii Officinalis Caulis. The content of monnose in Dendrobii Officinalis Caulis producted from different areas in Zhejiang Province has difference in quality and that produced from Jiande is the best.