Abstract: OBJECTIVE To investigate the clinical efficacy of acute cerebral infarction treated by nerve growth factor combined with acutobin injection. METHODS One hundred and sixty-eight patients with acute cerebral infarction were randomly divided into treatment group(n=68) and control group(n=68) according to admission time. Two groups were treated with conventional therapy including oral aspirin, the reasonable control of blood pressure, blood glucose, lipid, and reducing the intracranial pressure and complications and related complications treatment. The control group adopted intravenous injection of Xueshuantong 600 mg and acutobin injection 0.75 U added to 0.9% sodium chloride injection 250 mL, once a day, for 14 days. The treatment group in the control group based on the use of mouse nerve growth factor 18 μg, intramuscular injection, once a day, for 14 days. The changes of American national institutes of health stroke scale(NIHSS) score and the Oxford disability score(OHS), the markedly effective rate and total effective rate of treatment, and the side effects of 2 groups of patients were observed. RESULTS The efficacy of the treatment group was significantly superior to the control group(P<0.05). Before treatment, there was no statistically significant of the NIHSS score and OHS score between treatment group and control group; On the 21th day of treatment, the the NIHSS score and OHS score of treatment group was significantly better than that of the control group. At the twenty-first day of treatment, the treatment group obvious effective rate(72.1%) and total effective rate(94.1%) were significantly higher than that of the control group(respectively 58.8% and 86.8%). CONCLUSION Mouse nerve growth factor combined with acutobin injection more effectively improves the nerve function of patients with acute cerebral infarction, and is superior to the single use of acutobin injection.