迷迭香酸减少RhoA的表达抑制宫颈癌细胞的转移能力的研究

Rosmarinic Acid Inhibiting Metastasis of Cervical Cancer by Reducing RhoA Expression

摘要: 目的研究迷迭香酸对宫颈癌细胞转移能力的抑制作用及其可能机制。方法用1，5和10 μmol·L^-1的迷迭香酸分别处理宫颈癌细胞，通过Transwell小室法检测细胞的转移能力、半定量RT-PCR法检测细胞RhoA mRNA的表达、免疫荧光法检测RhoA蛋白的表达情况。结果 5，10 μmol·L^-1的迷迭香酸可以抑制宫颈癌细胞的转移(P<0.01)，而且2组细胞表达RhoA明显减少(P<0.05)。结论迷迭香酸可以抑制宫颈癌细胞的转移能力，其作用机制可能与减少细胞RhoA的表达有关。

Abstract: OBJECTIVE To investigate the inhibiting effect of rosmarinic acid on metastasis of cervical cancer and the possible mechanism. METHODS Cervical cancer cells were treated by rosmarinic acid with concentration of 1, 5, 10 μmol·L^-1. Transwell chamber was used to examine the metastasis of the cultured cells; semi-quantitative RT-PCR method was used to detect the expression of RhoA mRNA and immunofluorescence technique was used to detect the expression of RhoA protein. RESULTS The metastasis of cervical cancer was inhbited by rosmarinic acid with the concentration of 5, 10 μmol·L^-1 (P<0.01), and the expression of RhoA decreased significantly in the two groups(P<0.05). CONCLUSION Rosmarinic acid can inhibit the metastasis of cervical cancer, and the mechanism may be related to reducing the expression of RhoA in cells.