Abstract: OBJECTIVE To investigate microRNAs(miRNAs) derived from Zea mays and their target gene function. METHODS MiRNA profiles of Zea mays, in plasma of normal mice and Zea mays extract-treated mice were detected by using next generation sequencing, and then the miRNAs which were took into the organism were validated with miRNA database of Zea mays. Human mRNA database as targets was used to predicte target genes of miRNAs by TargetScan and MiRanda analysis, while the target gene function was considered by Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) analysis. RESULTS Twelve miRNAs were identified. For the KEGG pathway, their target gene mainly involved in insulin signaling pathway, MAPK pathway and peritumoral pathways by GO and KEGG analysis. The results of miRNAs-gene-network showed that miR4995, miR156a, miR396e might be the main pharmacodynamic substance. CONCLUSION MiRNAs might act as a bioactivity substance in the Zea mays.