Abstract: OBJECTIVE To explore the effect of Dahuang combined with live binary Bacillus subtilis on tumor necrosis factor alpha (TNF-α) and interleukin-6(IL-6) in rat with acute suppurative peritonitis. METHODS Sixty Wistar rats were randomly divided into four groups: blank control group, model group, live binary Bacillus subtilis group and Dahuang combined with live binary Bacillus subtilis group. The concentrations of TNF-α, IL-6 and white blood cell were compared among the groups. RESULTS The concentrations of serum TNF-α, IL-6 and white blood cell after 72 h modeling in the live binary Bacillus subtilis group and Dahuang combined with live binary Bacillus subtilis group were significantly lower than that after 24?h modeling(P<0.05). After 72 h modeling, the concentrations of serum TNF-α, IL-6 and white blood cell in the Dahuang combined with live binary bacillus subtilis group were significantly lower than that in the live binary bacillus subtilis group(P<0.05). The concentrations of TNF-α and IL-6 of lung, kidney, peritoneum, liver and small intestine in the Dahuang combined with live binary bacillus subtilis group after 72 h modeling were significantly lower than that in the live binary bacillus subtilis group(P<0.05). CONCLUSION Dahuang combined with live binary bacillus subtilis could reduce the level of TNF-α and IL-6 in rat with acute suppurative peritonitis.