Abstract: OBJECTIVE To research the distribution and accumulation of pulchinenosides(B3, BD, B7, B10, B11) in rats. METHODS The tissue distribution had been investigated after 300 mg·kg^-1 oral administration of pulsatilla chinensis (bunge) regel saponins(PRS) evenly dispersed suspension by RRLC-ESI-MS/MS. RESULTS PRS exhibited rapid and extensive distribution, and high level concentrations could be detected in rats’ tissues after 0.25 h for oral administration. T_max of 5 components in the tissues were about 30 min. The concentrations declined gradually after 2 h and a large proportion of PRS were almost eliminated after 6 h. PRS could also be detected in the brain. CONCLUSION PRS is difficult to accumulate in vivo. The medicine mainly distributes in heart, liver, lungs, spleen and small intestine, and it is highest in the heart than in other tissues, and it can transmitte across blood-brain barrier.