Abstract: OBJECTIVE To study the protective effect of Angelicae Sinensis Radix volatile oil on pituitrin-induced acute myocardial ischemic injury in mice. METHODS The mice were randanly divided into normal group, model group, high-, low-dose of Angelicae Sinensis Radix volatile oil, and Yinxingye pian group. The acute myocardial ischemic injury model was made by injecting pituitrin in mice, the height of the J spot was measured by electrocardiogram, serum was afforded for biochemical detections, the heart were fixed for pathological investigation to observe the degree of myocardial ischemic injury. RESULTS Compared with the model group, the moving up of J spots in the treated groups was significantly inhibited(P<0.01); the activities of SOD, GSH-Px and CAT increased, while the content of LD and MDA decreased(P<0.05); Nagar-Olsen staining showed that high- and low-dose of Angelicae Sinensis Radix volatile oil significantly diminished the areas of cardiac muscles injured by ischemia. CONCLUSION Angelica volatile oil has protective effect on acute myocardial ischemic injury in mice.