Abstract: OBJECTIVE To investigate the effect of voriconazole on the hypoglycemic effect of non-sulfonylureas in nomal and streptozotocin(STZ)-induced diabetic rats. METHODS ①nomal rats were randomly divided into 2 groups: control group and voriconazole group. The later group animals received voriconazole intragastricly for 7 consecutive days. On 8th day, all the animals were fasted for 8 h, and blood samples were drawn from orbit at regular interval of 0, 1, 3, 6, 9, 12, 18, 24 h. Then fasting blood glucose was measured. ②Nomal rats were randomly divided into 4 groups: group Ⅰ rats just received repaglinide, and group Ⅱ rats received repaglinide and voriconazole. Group Ⅲ rats received nateglinide, and group Ⅳ rats received nateglinide and voriconazole. Group Ⅱ and Ⅳ rats received voriconazole intragastricly for seven consecutive days. On 8th day, all the animals were fasted for 8 h, and blood samples were drawn from orbit at regular interval of 0, 1, 3, 6, 9, 12, 18, 24 h. Then fasting blood glucose was measured. ③Wistar rats received STZ to induce diabetes mellitus. The methods of grouping and giving drug were the same as the second part of experiments. RESULTS Therapeutic dose of voriconazole enhanced the hypoglycemic effect of repaglinide and nateglinide in nomal and diabetic rats. CONCLUSION The results indicate that the dose of non-sulfonylureas needs to be adjusted if co-administered with voriconazole.