Abstract: Since entering the 21st century, drug screening technology is developing fast and efficiently towards to the cellular level screening. As a promising platform for the exploration of modern life science, microfluidic chip technology, with its analysis of miniaturization, qualcomm quantitative, can be integrated and good biocompatibility, etc, has attracted increasing attention on cell-based drug screening, and adopted a series of achievements. In the 12th five-year plan, our country started the science and technology major projects, making microfluidic chip reached a new height in the field of drug screening. In this paper, the microfluidic chip system based on a cellular level of drug screening in recent years are reviewed. In addition, the further applications of microfluidic chip are prospected based on the current development．