Abstract: OBJECTIVE To investigate the percutaneous permeability of liranaftate spray. METHODS The improved Franz diffusion cells and the skin of Bama miniature pig were used to investigate the percutaneous permeability of liranaftate. The content of liranaftate was determined by HPLC. RESULTS The steady penetration rate of spray and cream was (0.017±0.006), (0.014±0.003)μg·cm^-2·h^-1, respectively. The 24 h cumulative permeation quantity of spray and cream was (0.60±0.23), (0.44±0.15)μg·cm^-2, respectively. The residue in skin of spray and cream was (8.2±1.3), (7.7±1.6)μg·g^-1, respectively. CONCLUSION Although liranaftate spray shows slightly higher steady penetration rate, 24?h cumulative permeation quantity, and residue than liranaftate cream, there is no significant difference of the steady penetration rate, 24 h cumulative permeation quantity and residue between liranaftate spray and liranaftate cream.