Abstract: OBJECTIVE To investigate the effects of rutin on oxidative stress of acute lung injury induced by lipopolysaccharide(LPS). METHODS Fifty male C57 mice were randomly divided into five groups: control group, model group, and rutin low, middle, high(25, 50, 100 mol·kg^-1) groups. After 6 h of LPS injected, collecting arterial blood for blood gas analysis and lung tissue to examine pathological change, Wet/Dry, the expression of MDA and the activity of CAT, GPx and SOD. RESULTS Compared with the control group, the pathological change and Wet/Dry of lung tissue, the p(CO₂) and respiratory rate(RR), the expression of MDA in lung tissue were dose-increased, while the p(O₂), pH and the activity of CAT, GPx and SOD were decreased. compared with the model group, the pathological change and Wet/Dry of lung tissue, the p(CO₂) and RR, the expression of MDA in lung tissue in rutin groups were concentration-dependent decreased, while the p(CO₂), pH and the activity of CAT, GPx and SOD were concentration-dependent increased. CONCLUSION Rutin pretreatment can concentration- dependent decrease LPS induced acute lung injury by suppressing oxidative stress.