Abstract: OBJECTIVE To establish the fingerprint chromatogramand multi-component quantification analysis method of Leonurus liquid extract. METHODS Extracted by n-Buthyl alcohol, the extract was analyzed on Kromasil C₁₈ column (250 mm×4.6 mm, 5 μm)and the temperature of column was 30 ℃. The mobile phase consisted of acetonitrile-0.1% formic acid with gradient elution. The flow rate was 1.0 mL·min^-1. The detection wavelength was at 275 nm. RESULTS HPLC fingerprint of characteristic components of Leonurus liquid extract was established for the first time, 6 common peaks were figured out, and 3 of them were identified and determinated. P-hydroxybenzoic acid, syringic acid, and hydrochloric acid leonurine showed good linearities in the ranges of 3.952-790.4 ng(r=1.000 0), 10.427-2 085.4ng(r=1.000 0) and 6.983-1 396.6 ng(r=0.999 9), the average recoveries were 98.6%, 97.8% and 97.9%, with RSD of 1.5%, 2.0% and 1.8%, respectively. CONCLUSION The HPLC characteristic fingerprint for the identification of Leonurus liquid extract was specific, simple and reproducible. The established multi-component quantification analysis method could be used to control the quality of Leonurus liquid extract, and provided the basis of comprehensive evaluation and quality control.