Abstract: OBJECTIVE To establish the portability cervical cancer (U27) tumor mice model and to study the influence of Abnormal Savda Munziq (ASMq) on serum metabolism of cervical cancer (U27) tumor model mice. METHODS Using Nuclear Magnetic Resonance Spectroscopy (¹H-NMR) technology combined with pattern recognition and orthogonal partial least squares discriminant analysis (OPLS-DA) method analyze to seeking for the variability of serum metabolites and metabolic network pathways of cervical cancer (U27) tumor mice’s after oral administration of low, medium, high dose of ASMq and cyclophosphamide drug intervention. RESULTS Compared with normal control group, the concentration level of some metabolites were reduced in the cervical cancer (U27) tumor model group mice’s serum, including isoleucine, valine, alanine, glutamine, glycine, amino acid and lactic acid, citric acid, creatine and 1-methyl histidine; while low density lipoprotein level was increased(P<0.05). Compared with the cervical cancer (U27) tumor model group, leucine and alanine levels was increased in the cyclophosphamide control group(P<0.05); after oral administration the different doses of ASMq, the serum in the cervical cancer (U27) tumor model group mice’s, the amino acids that isoleucine, leucine, valine, alanine, glutamine content was increased and glycoprotein, citric acid, creatine content also had a rising trend(P<0.05). CONCLUSION ASMq has some antitumor effects on cervical cancer (U27) tumor mice model, and it’s mechanism of prevention and treatment of tumor may be achieved not only by adjusting the disordered amino acid metabolism and energy metabolism, but also improving the immune function of cervical cancer (U27) tumor model mice.