Abstract: OBJECTIVE To investigate the Optimum conditions of microemulsion ultrasonic method in extracting ganoderic acid and polysaccharides of antler-shape Ganoderma lucidum. METHODS Central composite design and response surface methodology were used to optimize the extraction. The ratio of water to material(X₁), ultrasonic extracting time(X₂) and ultrasonic power(X₃) were selected as examine factors, whereas, the ganoderic acids A and C₂, the Ganoderma lucidum polysaccharide extraction content and the amount of extraction were set as evaluation targets. Multiple linear regression and binomial fitting were used to estimate the relationship between overall desirability and examine factors. Response surface methodology was applied to optimize the process of microemulsion ultrasound extraction. Confirmatory tests were carried out in the end. RESULTS The best condition of microemulsion ultrasonic method in extraction of the antler-shape Ganoderma Lucidum was that the ratio of water to material was 18, ultrasonic extracting time was 59 min, ultrasonic power was 513 W. Under this condition, the ganoderic acids A and C₂, the Ganoderma lucidum polysaccharide extraction content was respectively 0.647 8, 0.108 5, 11.20 mg·g^-1 and the amount of extraction was 34.28%. CONCLUSION Mcroemulsion ultrasonic method provides a new way for the industrial extraction of antler-shape Ganoderma lucidum. It can extract water and fat soluble components simultaneously, moreover, it is a simple energy-saving, time-saving and operations method.