Abstract: OBJECTIVE To investigate the protective effect of melatonin on irritability gastric mucosa injury in rats of cerebral hemorrhage. METHODS All 60 adult healthy male Sprague-Dawley rats were randomly divided into blank control group, model group, omeprazole group, melatonin group. The rats received respective treatments by prophylactic administration for 7 d: the blank control group and model group received normal saline by gavage; the omeprazole group received water suspension of omeprazole by gavage; melatonin group received melatonin by gavage. After the last administration, followed by 24 h of fasting(without water deprivation), a rat model of cerebral hemorrhage was established in all groups except blank control, After the model was established, serum levels of MDA, SOD were determined by enzyme-linked immunosorbent assay, gastric morphology was observed; gastric ulcer index (UI) was calculated; and the gastric mucosal tissue HE staining microscopy results and the incidence of gastric mucosal injury were compared. RESULTS Melatonin increased the SOD content and reduced MDA content, which reduced the rat gastric mucosa UI and the incidence of gastric mucosal injury (P<0.05). CONCLUSION Melatonin may protect stress gastric mucosal through clear oxygen free radicals.