Abstract: OBJECTIVE To establish an HPLC method for the determination of the content and content uniformity of atropine sulfate tablets. METHODS SHISEIDO CAPCELL C₁₈ chromatographic column(250 mm×4.6 mm, 5 μm) was adopted. The mobile phase was 0.01 mol·L^-1 potassium dihydrogen phosphate solution(including 0.15% triethylamine, adjusted to pH 6.0 by phosphoric acid)-methanol(75∶25) at the flow rate of 1.0 mL?min^-1. The detection wavelength was 210 nm, and the column temperature was 35 ℃. The injection volume was 20 μL. RESULTS Good linear correlation was found in the range of 5.878 8-58.788 μg·mL^-1, and the linear equation was Y=24.015 8X+0.095 9, r=1.000 0. The average recovery was 100.2%(n=9). CONCLUSION This method is simple, rapid and efficient for the quality control of atropine sulfate tablets.