Abstract: OBJECTIVE To develop a method for total solid content determination of Shuxuetong injection in lixivating process based on near-infrared spectroscopy. METHODS Taking the lixivating process of Hirudo and Earthworm for examples, the quantitative calibration models for the total solid content were established by partial least squares (PLS). And the established PLS calibration models were used to rapidly analyze the total solid content in lixivating process of Shuexuetong injection. RESULTS Optimal models were obtained when raw spectra were processed with first derivative combined with Karl Norris smoothing. The correlation coefficient of total solid content was 0.810 8 for lixivating process of Hirudo. Root mean square error of calibration and prediction were 0.583 and 0.495, respectively. Root mean square error of cross validation was 0.81, and relative standard errors of calibration and prediction was 6.11% and 5.25%, respectively. The correlation coefficient of total solid content was 0.975 5 for lixivating process of Earthworm. Root mean square error of calibration and prediction were 1.10 and 1.85, respectively. Root mean square error of cross validation was 1.61, and relative standard errors of calibration and prediction was 4.68% and 7.80%, respectively. CONCLUSION NIR spectroscopy was successfully applied to determine total solid content in lixivating process of Hirudo and Earthworm. It can be used for the quality control in manufacturing process of Shuxuetong Injection.