Abstract: OBJECTIVE To establish an HPLC method to simultaneously determine three kinds of flavonoids in Chimonanthus zhejiangensis M. C. Liu. METHODS The Agilent Zorbax SB-C₁₈ chromatographic column (4.6 mm×250 mm, 5 μm) was adopted. The column temperature of was 30 ℃. The mobile phase was acetonitrile-0.1% phosphoric acid solution with the gradient elution at the flow rate of 1.0 mL·min^-1. The detection wavelength was 360 nm. RESULTS Good linear correlation of rutin, quercetin and kaempferol were found in the ranges of 4.057-507.2, 0.686 1-171.5, 0.216 9-81.35 μg·mL^-1(r=0.999 3, 0.999 6, 0.999 9), respectively. The average recoveries were 99.97%(RSD=0.97%), 100.13%(RSD=1.06%), 99.35%(RSD=1.39%). CONCLUSION This method is simple, accurate and repeatable for simultaneously determination of the contents of rutin, quercetin and kaempferol in Chimonanthus zhejiangensis M. C. Liu, and applicable for the quality control of Chimonanthus zhejiangensis M. C. Liu.