HPLC同时测定冠心康颗粒中芍药苷、丹酚酸B和羟基红花黄色素A的含量

喻录容; 何先元; 黄英如

HPLC同时测定冠心康颗粒中芍药苷、丹酚酸B和羟基红花黄色素A的含量

Simultaneous Determination of Paeoniflorin, Salvianolic Acid B and Hydroxysafflor Yellow A in Guanxinkang Granules by HPLC

摘要

摘要: 目的建立同时测定冠心康颗粒中芍药苷、丹酚酸B和羟基红花黄色素A含量的方法。方法采用Welchrom C₁₈色谱柱(250 mm×4.6 mm，5 μm)，以乙腈(A)-0.1%磷酸(B)为流动相，梯度洗脱；流速：1.0 mL·min^-1；检测波长：230 nm。结果芍药苷在7.02~52.64 μg·mL^-1内呈良好的线性关系(r=0.999 7)，平均回收率为99.3%，RSD为1.2%；丹酚酸B在23.19~173.90 μg·mL^-1内呈良好的线性关系(r=0.999 7)，平均回收率为98.2%，RSD为1.1%；羟基红花黄色素A在4.47~33.50 μg·mL^-1内呈良好的线性关系(r=0.999 3)，平均回收率为98.0%，RSD为1.0%。结论本法简便、灵敏、准确、重复性好，可用于冠心康颗粒的质量控制。

Abstract: OBJECTIVE To establish a simultaneous determination method for paeoniflorin, salvianolic acid B and hydroxysafflor yellow A in Guanxinkang granules. METHODS The determination was performed on a Welchrom C₁₈ column(250 mm×4.6 mm, 5 μm) with acetonitrile(A)-0.1% phosphoric acid(B) as the mobile phase in gradient elution mode at the flow rate of 1.0 mL·min^-1. The detection wavelength was 230 nm. RESULTS Paeoniflor presented a good linearity in the concentration range of 7.02-52.64 μg·mL^-1. The average rate of recovery was 99.3 % with RSD of 1.2%. Salvianolic acid B presented a good linearity in the concentration range of 23.19-173.90 μg·mL^-1. The average rate of recovery was 98.2% with RSD of 1.1%. Hydroxysafflor yellow A presented a good linearity in the concentration range of 4.47-33.50 μg·mL^-1. The average rate of recovery was 98.0% with RSD of 1.0%. CONCLUSION This method was simple, accurate and reproducible, it can be used as the basis for quality control of Guanxinkang granules.

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