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    HPLC测定三七提取液中三七皂苷R2(S)、七叶胆苷XVII和人参皂苷F2的含量

    Determination of Notoginsenoside R2(S), Gypenoside XVII and Ginsenoside F2 in Notoginseng Radix et Rhizoma Extracts by HPLC

      摘要
    • 摘要: 目的 建立同时测定三七提取液中三七皂苷R2(S)、七叶胆苷XVII及人参皂苷F2含量的高效液相色谱法。方法 色谱柱为Waters Acquity UPLC CSH-C18(50 mm×2.1 mm，1.7 μm)，流动相为0.01%甲酸-水(A)和0.01%甲酸-乙腈(B)，梯度洗脱，检测波长为203 nm，进样量为5 μL，流速为0.35 mL·min-1，柱温为40 ℃。结果 在43 min内可完成三七皂苷R2(S)、七叶胆苷XVII和人参皂苷F2的分离测定。3种皂苷峰面积和浓度线性关系良好(r2>0.999 8)；日内和日间精密度RSD<3.4%；回收率为98.4%~102.1%。结论 该方法简便、准确，重复性好，可用于三七提取液中皂苷成分的测定。

       

      Abstract: OBJECTIVE To develope an HPLC method for the simultaneous determination of notoginsenoside R2(S), gypenoside XVII and ginsenoside F2 in Notoginseng Radix et Rhizoma extracts. METHODS The analysis was performed on an Agilent 1260 series HPLC system with Waters Acquity UPLC CSH-C18 (50 mm×2.1 mm, 1.7 μm) column. Gradient elution using 0.01%-formic acid-water and 0.01%-formic acid-acetonitrile as the mobile phases with detection wavelength of 203 nm, flow rate of 0.35 mL·min-1, injection volume 5 μL and column temperature 40 ℃ was applied. RESULTS The total analyzing time was 43 min. The high determination coefficient values (r2>0.999 8) indicated good correlations between saponin concentrations and their peak areas within the test ranges. The overall intra-day and inter-day variations (RSD) of 3 major saponins were <3.4%. The developed method was successfully used for the analysis of saponins in Notoginseng Radix et Rhizoma extracts with overall recovery of 98.4%–102.1% for all the analytes. CONCLUSION This analytical method is accurate and convenient for the determination of 3 saponins in Panax notoginseng extracts.

       

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