Abstract: OBJECTIVE To evaluate the effect of the methicillin-resistant staphylococcus aureus (MRSA) therapeutic drug application by means of drug use evaluation by clinical pharmacists. METHODS choose 220 cases using anti-MRSA drugs from Jan to May, 2013 (before intervention) (including vancomycin, teicoplanin, linezolid) and 226 cases from Aug to Dec (after the intervention), analyze with the combination of drug use evaluation criteria for anti-MRSA drugs. RESULTS The rational use of anti-MRSA drug has been improved greatly with intervention, Number of unreasonable using cases before intervention was up to 138, but reduced to 85 cases after intervention. CONCLUSION our clinical pharmacists, who intervented the application of anti-MRSA drugs in a short term by means of drug utilization evaluation, have achieved initial success. Clinical pharmacists can timely discover and solve the problems and give comprehensive evaluation of rational use of anti-MRSA drugs to improve the rational application of these drugs and medical quality.