Abstract: OBJECTIVE To investigate the quality of the 16 batches of commercial Aurantii Fructus by establishment of HPLC fingerprinting. METHODS HPLC was applied with the chromatographic condition as follows: the chromatographic column was Agilent Zorbax SB-C₁₈ (250 mm×4.6 mm, 5 μm) at 30 ℃; mobile phase was acetonitrile-water(20∶80)(adjusted to pH 3 with phosphoric acid); flow rate was 1.0 mL?min^-1, the detection wavelength was 283 nm. Used the similarity evaluation system for fingerprint similarity calculation. RESULTS Sixteen batches of commercial Aurantii Fructus were reaching pharmacopoeia standards, there were a little differences between two processing methods. Fingerprint similarity was above 0.98. CONCLUSION The method of fingerprint is stable, repeatable and it is suitable for the quality control of Aurantii Fructus.