Abstract: OBJECTIVE To prepare sophoridine cationic liposome, and study its inhibitory effect on tumor cells. METHODS Active-loading method was used to prepare sophoridine liposomes, their characterization were investigated, futher, MTS method was used to evaluate the inhibitory effects of sophoridine cationic liposome on 3 tumor cells. RESULTS The sophoridine cationic liposomes which had been prepared were round with smooth surface, their average particle size, PDI, surface charge, encapsulation efficiency and drug loading amount was 242.2 nm, 0.180, +32.5 mV, 88.62% and 5.97% respectively. The IC50 of sophoridine cationic liposome on 3 tumor cells were obviously lower than sophoridine, and the blank cationic liposomes had no obvious inhibitory effect to cells. CONCLUSION Using cationic liposome as carrier for Sophoridine makes the drugs through the cell membrane easier, and improves the anti-tumor effect, is worth in-depth research in future.