Abstract: OBJECTIVE To establish a method for contaminating microorganisms test in Bacillus licheniformis particles. METHODS Spread plate method was used in the control bacteria examination; the non-pathogenic microbes was counted in nutrient agar and growth at 37 ℃, the maximum acceptable count was suggested in 0.1% of total microbes; the sodium rose Bengal agar and growth at 25 ℃ was used to do the fungi count. RESULTS The method was accepted in validation test of Chinese pharmacopoeia 2010. CONCLUSION The method can be used in contaminants test for Bacillus licheniformis particles control.