Abstract: OBJECTIVE To prepare a liposome hydrogel taxol, study the analgesic and anti-inflammatory effects. METHODS Taxol-liposome gel was obtained by thin film dispersion method. The shape, size, size distribution and encapsulation efficiency were detected by transmission electron microscope(TEM), nano-particle size analyzer and HPLC, respectively. The gel was then prepared based on the liposome. The in vitro release of taxol-liposome gel in different time was detected with transdermal experiments. Observe its effect on mouse inflammatory model induced by carrageenin and the pain model induced by formaldehyde. RESULTS The shape of taxol-liposome was regular and narrowly-distributed, and its encapsulation efficiency was (73.6±0.3)%. The prepared gel was semitransparent. After that, transdermal experiments, taxol-liposome gel met a kinetic equation, played better sustained release. In vitro transdermal experiments, after 12 h taxol-liposome through maximum amount, played better sustained action. In inflammatory experiment, compared with the model group, the taxol-liposome gel produced significant inhibition on rat paw edema in a dose-dependent manner(P<0.05). In mouse formalin test, taxol-liposome gel reduced the number of writhing induced by acetic acid, significantly(P<0.05). CONCLUSION The taxol-liposome gel has significant analgesic and anti-inflammatory effects.