Abstract: OBJECTIVE To establish a method for simultaneous determination of ten bioactive components in Qingqing granule by quantitative analysis of multi-components by single-marker(QAMS). METHODS Qingqing granule was chosen as the research object, baicalin as index, relative correction factors of gallic acid, albiflorin, paeoniflorin, liquiritin, glycyrrhizic acid, wogonoside, baicalein, wogonin and berberine were established by HPLC and calculated to achieve QAMS. The contents of 10 components in the samples were determined with the external standard method. The accuracy of the method was validated by compare the results of the above two methods. RESULTS No significant differences were found between the calculated results by QAMS and the measured results by external standard method. CONCLUSION The described method is reliable, accurate, and can be used to control the quality of ten bioactive components of Qingqing granule.